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KMID : 0616120230540010027
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Korean Journal of Pharmacognosy
2023 Volume.54 No. 1 p.27 ~ p.37
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Evaluation of Comprehensive Effects of Methanol Extracts of Coptidis rhizoma against Several Oral Pathogenic Bacteria
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Seo Chae-Hyeon
Song Young-Cheon
Yim Dong-Sool
Choi Sung-Sook
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Abstract
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This research was conducted to investigate the comprehensive effects of methanol extract of Coptidis rhizoma (MECR) against oral pathogen. We studied the antibacterial, anti-biofilm, anti-gingipain and anti-inflammatory activity of MECR. The minimum bactericidal concentration (MBC) of MECR was 100 μg/mL against several oral pathogens. The formation of biofilm of Streptococcus mutans was reduced to 8.93~24.12% in the presence of 25 μg/mL of MECR. The gingipain activity of Porphyromonas gingivalis were reduced to 3.91~6.23% in case of Kgp and 5.73~7.78% in case of Rgp in the presence of 10 mg/mL of MECR. The expression of fadA mRNA, virulence factor of Fusobacterium nucleatum (F. nucleatum) was 3 folds decreased in the presence of 25 μg/mL of MECR. In case of YD-38 cells challenged with F. nucleatum, RQ values of IL-8 and IL-6 were reduced about 12 folds and 5.45 folds in the presence of 2 μg/mL of MECR. In case of RAW 264.7 murine cell challenged with F. nucleatum, RQ values of IL-1β and IL-6 were 2.52 folds and 2.55 folds reduced in the presences of 2 μg/mL of MECR. Conclusively, MECR showed potent antibacterial and anti-inflammatory effects against oral pathogenic bacteria.
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KEYWORD
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Coptidis rhizoma, Oral pathogens, Antibacterial activity, Anti-biofilm, Anti-gingipain, Anti-inflammatory activity
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